Figure 2. Behavioural response to the fear conditioning paradigm.

Animals were trained with paired CS-US presentations (paired group (PG), open bars; n = 6) and unpaired CS and US presentations (unpaired group (UG), black bars; n = 6) and freezing behaviour was assessed 24 h later in a separate context. A two-way ANOVA revealed that PG spent a greater proportion of time freezing than UG animals during all three stimulus presentations (P<0.01). Data is represented as mean ± SEM.