Figure 2. Immunodetection of proteins in obeche wood dust.

2A. In-house obeche wood dust extract (40 µg; Obeche), purified protein (5 µg; 24 kDa; 12 kDa) or purified allergens Act d 2 and Prs a 1 (5 µg) were separated by SDS-PAGE and stained with Coomassie blue (Coomassie). Replicas from the extract were electrotransferred and incubated with serum pool of selected exposure subjects (anti-IgE) (2B) or with antibodies produced against chestnut thaumatin-like protein (anti-TLP) (2C), against chestnut chitinases (anti-Ch) (2D) or anti N-glycan antibodies (anti-CCD) with bromelain and bovine serum albumin (BSA) as positive and negative controls (2E).