Table 2. Frequency of JAK2 V617F mutation in Korean MPNs patients.

Methods	MPNs (No. of patients)					Total No. (%)
	Results	PV (26)	ET (42)	PMF (7)	UC (3)	78
Direct sequencing	Mutant	8	2	3	0	13
	Mixed	10	10	3	0	23
	Wild-type	8	30	1	3	42
	No (%)	18 (69.2)	12 (28.6)	6 (85.7)	0 (0)	36 (46.2)
REA	Mutant	7	2	3	1	13
	Mixed	4	4	3	0	11
	Wild-type	15	36	1	2	54
	No (%)	11 (42.3)	6 (14.3)	6 (86)	1 (33.3)	24 (30.8)
PSQ	Mixed	18	11	7	2	38
	Wild-type	8	31	0	1	40
	No (%)	18 (69.2)	11 (26.2)	7 (100)	2 (66.7)	38 (48.7)
qPCR	Mixed	24	17	6	2	49
	Wild-type	2	25	1	1	29
	No (%)	24 (92.3)	17 (40.5)	6(85.7)	2 (66.7)	49 (62.8)
AS- PCR	Mixed	23	24	6	2	55
	Wild-type	3	18	1	1	23
	No (%)	23 (88.5)	24 (57.1)	6 (85.7)	2 (66.7)	55 (70.5)
Overall*	No (%)	23 (88.5)	25 (59.5)	7 (100)	2 (66.7)	57 (73.1)

REA, restriction enzyme-based assay; PSQ, pyrosequencing; AS-PCR, allele-specific PCR with DPO primer; other Abbreviations as in Table 1.

^*, calculated from the number of patients having at least one method with positive result.