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Bacterial strains were inoculated into TMH without calcium and incubated at 26°C to an OD600nm of 1.0. The bacterial cultures were then transferred to 37°C for 3 hours incubation to induce the Yops expression and secretion. Proteins from the culture supernatant (S) and the cell pellets (P) were separated by SDS-PAGE electrophoresis and detected using rabbit polyclonal antibodies against YopJ and YopM.
