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. Author manuscript; available in PMC: 2013 Sep 25.
Published in final edited form as: Biochemistry. 2012 Sep 11;51(38):7444–7455. doi: 10.1021/bi300491j

Figure 2.

Figure 2

TBP/DNA complexes exist in a uniform FRET state. (A) Consensus TATA DNA exists in a homogenous population in a relatively low FRET state. FRET efficiencies for 301 DNA molecules were plotted as a histogram and fit with a Gaussian. The mean FRET efficiency is 0.24. (B) In the presence of TBP, the DNA molecules exist in a two-state population (unbent and bent). Time traces for 477 DNA molecules were analyzed and 1727 distinct FRET occurrences were plotted as a histogram and fit with Gaussians. The low and high FRET states have mean FRET efficiencies of 0.26 and 0.39, respectively. (C) Shown is the histogram and Gaussian fit of FRET efficiencies determined for 72 TATA-less DNA molecules in the absence of TBP. The mean FRET efficiency is 0.23. (D) TBP does not alter the FRET efficiency of a TATA-less DNA construct. The histogram and Gaussian fit of 124 distinct FRET occurrences observed in time traces from 121 TATA-less DNA molecules in the presence of TBP is shown. The mean FRET efficiency is 0.24.