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. Author manuscript; available in PMC: 2013 Sep 25.
Published in final edited form as: Biochemistry. 2012 Sep 11;51(38):7444–7455. doi: 10.1021/bi300491j

Figure 4.

Figure 4

TFIIA facilitates TBP bending the mutant TATA(A3) sequence. (A) In the absence of TBP, the mutant TATA sequence exists in a uniform FRET state similar to that observed with the consensus TATA sequence. The mean FRET efficiency for the Gaussian fit of data from 206 DNA molecules is 0.25. (B) TBP does not shift the TATA(A3) DNA into a higher FRET state. Shown is the histogram and Gaussian fit of 427 distinct FRET occurrences observed in time traces from 414 TATA(A3) DNA molecules in the presence of TBP; the mean FRET efficiency is 0.26. (C) TFIIA facilitates the bending of the TATA(A3) DNA by TBP. The mean FRET efficiencies of the low (319 occurrences) and high (274 occurrences) states obtained from the Gaussian fits of distinct FRET occurrences observed in time traces from 400 TATA(A3) molecules are 0.27 and 0.41, respectively.