Figure 7.
Unbending on the TATA(A3) DNA in monophasic. (A) Analysis of the rate at which TBP bends the TATA(A3) construct. Dwell times for the unbent occurrences observed at 50 mM KCl were histogrammed and fit to a monoexponential. The observed rate constant is 0.12 ± 0.03 s−1, where the error represents the 95% confidence interval (B) Unbending of the TATA(A3) construct. Dwell times for the bent occurrences observed at 50 mM KCl were histogrammed and fit to a monoexponential. Residuals are plotted below the rate plot. The rate constant obtained is 0.31 ± 0.04 s−1, where the error represents the 95% confidence interval