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. Author manuscript; available in PMC: 2013 Sep 25.
Published in final edited form as: Biochemistry. 2012 Sep 11;51(38):7444–7455. doi: 10.1021/bi300491j

Table 2.

Mean FRET efficiencies.

DNA construct [KCl] mM TBP TFIIA Unbent mean FRET Bent mean FRET Unbent 95%CI Bent 95%CI
Cons. TATA 150 0.24 N/A 0.241–0.244 N/A
Cons. TATA 150 + 0.26 0.39 0.261–0.266 0.388–0.394
Cons. TATA 150 + + ND 0.39 ND 0.386–0.398
Cons. TATA 50 0.4x 0.26 0.37 0.256–0.271 0.363–0.380
TATA(A3) 150 0.25 N/A 0.249–0.253 N/A
TATA(A3) 150 + 0.26 ND 0.259–0.262 ND
TATA(A3) 150 + + 0.27 0.41 0.271–0.277 0.402–0.411
TATA(A3) 50 0.25 N/A 0.250–0.258 N/A
TATA(A3) 50 + 0.27 0.36 0.262–0.274 0.353–0.369
TATA(A3) 50 4x 0.26 0.37 0.253–0.271 0.361–0.379
TATA-less 150 0.23 N/A 0.224–0.231 N/A
TATA-less 150 + 0.24 ND 0.239–0.244 ND

N/A – not applicable

ND – not detected

95% CI – 95% confidence interval for the mean FRET