Table 2.
DNA construct | [KCl] mM | TBP | TFIIA | Unbent mean FRET | Bent mean FRET | Unbent 95%CI | Bent 95%CI |
---|---|---|---|---|---|---|---|
Cons. TATA | 150 | − | − | 0.24 | N/A | 0.241–0.244 | N/A |
Cons. TATA | 150 | + | − | 0.26 | 0.39 | 0.261–0.266 | 0.388–0.394 |
Cons. TATA | 150 | + | + | ND | 0.39 | ND | 0.386–0.398 |
Cons. TATA | 50 | 0.4x | − | 0.26 | 0.37 | 0.256–0.271 | 0.363–0.380 |
TATA(A3) | 150 | − | − | 0.25 | N/A | 0.249–0.253 | N/A |
TATA(A3) | 150 | + | − | 0.26 | ND | 0.259–0.262 | ND |
TATA(A3) | 150 | + | + | 0.27 | 0.41 | 0.271–0.277 | 0.402–0.411 |
TATA(A3) | 50 | − | − | 0.25 | N/A | 0.250–0.258 | N/A |
TATA(A3) | 50 | + | − | 0.27 | 0.36 | 0.262–0.274 | 0.353–0.369 |
TATA(A3) | 50 | 4x | − | 0.26 | 0.37 | 0.253–0.271 | 0.361–0.379 |
TATA-less | 150 | − | − | 0.23 | N/A | 0.224–0.231 | N/A |
TATA-less | 150 | + | − | 0.24 | ND | 0.239–0.244 | ND |
N/A – not applicable
ND – not detected
95% CI – 95% confidence interval for the mean FRET