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. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: J Immunol. 2012 Dec 26;190(3):1169–1179. doi: 10.4049/jimmunol.1202353

Figure 6.

Figure 6

CD70 is dispensable for recall responses. (A) The experimental scheme is depicted. CD45.1+P14 TCR Tg transgenic CD8 T cells were adoptively transferred to a WT recipient (CD45.2+), which was then infected with LCMV. After 1–2 months memory P14 cells were sorted and adoptively transferred into WT or CD70−/− recipients. The recipient mice were subsequently infected with LCMV and the recall response was evaluated five or seven days later. (B–D) Absolute numbers of adoptively transferred memory P14 CD8 T cells (B), GP33-tetramer+ cells (C) and endogenous effector CD8 T cells (D) in WT or CD70−/− mice after five or seven days of infection with LCMV are depicted. Mean ± SEM of three-five mice of each strain per time point is shown. (E) PD-1, KLRG1 and Annexin V surface levels on CD8 T cells of uninfected (filled histogram), P14-Tg cells in WT (thin black line), or P14-Tg cells in CD70−/− (black thick line) splenocytes is depicted. (F–G) WT or CD70−/− mice were infected with LCMV and 8 months later rechallenged in vivo with GP33 peptide by footpad administration. Absolute numbers of GP33-tetramer+ T cells in draining and non-draining lymph nodes (F) and in spleen (G). Mean ± SEM of three mice is shown.