Fig. 2. TLR7.1 myeloid progenitors exhibit a type I IFN signature in the bone marrow where pDC are constitutively activated and generating type I IFN mRNA.

(A) ISG mRNA from BM progenitors, sorted as in Fig. 1A and assayed by qPCR. Data are from 2 independent experiments. (B) BM pDC from WT and TLR7.1 mice were gated on as CD11b⁻Siglec-H⁺BST-2⁺ and a histogram of MHCII expression is shown, representative of 5 independent experiments. (C) BM pDC from mixed BM chimeras generated with a 1:1 ratio of TLR7.1 and B6.SJL BM were gated as CD45.2⁺ and CD45.1⁺, respectively. MHCII expression on CD45.2⁺ and CD45.1⁺ BM pDC from the same mouse are shown, representative of 3 independent experiments. (D) BM cells were sorted (pDC as in Fig. 2B; inflammatory monocytes CD11b⁺Ly6C⁺Ly6G^-; neutrophils CD11b⁺Ly6C⁺Ly6G⁺) and used for qPCR. Data are representative of two experiments. n.d. not detected.