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. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: Virus Res. 2012 Sep 24;171(2):287–303. doi: 10.1016/j.virusres.2012.09.008

Figure 2. Complexes formed between Vpr and dsDNA observed by TEM in dark field mode.

Figure 2

Reaction were carried out for 15 min. at 37 °C in 25mM HEPES (pH7.5), 50mM sodium acetate, 6mM magnesium acetate. a-d) Vpr:dsDNA nucleofilaments formed after incubation of pTZ18R DNA (2.5nM) and Vpr (1μM). (a) typical double-lasso structures bearing a synaptic joint; b) wire-like structure upon full coverage; c) plasmid spread by Vpr. d) is a zoom of e) with inverted contrast to better see the protein shell on DNA strands (arrows). e-g) Complexes obtained with Vpr and 1.68kbp linear dsDNA, for respectively a bp/Vpr ratio of 16, 12 and 10. The scale bars represent 80 nm.