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. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: Virus Res. 2012 Sep 24;171(2):287–303. doi: 10.1016/j.virusres.2012.09.008

Figure 3. TEM visualization of RNA-dsDNA or ssDNA-dsDNA mixtures, incubated with NCp7 or with NCp7 and Vpr.

Figure 3

Reaction were carried out for 15 min. at 37 °C in 25mM HEPES (pH7.5), 50mM sodium acetate, 6mM magnesium acetate. (a) Incubation of 2.5 nM dsDNA (pTZ18R) and 5 nM ssDNA (circular, 3.3 kbp) with 3.4μM NCp7 (nucleotide/NCp7 ratio: 10). (b) Incubation of 3.4μM NCp7 plus 3μM Vpr with 2.5 nM dsDNA and 5nM ssDNA (nucleotide/NCp7 and /Vpr ratio: 10). (c-d) Incubation 30min. at 37°C of 3.4μM NCp7 plus 3μM Vpr with 2.5 nM dsDNA and 5nM polyrA (nucleotide/NCp7 and/Vpr ratio: 10). Complexes were diluted 5-fold in the reaction buffer before deposition on the TEM grid. Arrows point Vpr:dsDNA nucleofilaments. The scale bars represent 80 nm.