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. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: Aging Cell. 2012 Dec 6;12(1):121–129. doi: 10.1111/acel.12027

Fig 3.

Fig 3

Effect of targeted manipulation of β-arrestin abundance in the IPCs. (A) Relative suppression of β-arrestin abundance by IPC-specific β-arrestin RNAi compared with control strain (w1118 x Dilp2-GAL4). Simultaneous IPC-specific overexpression of mth and suppression of IPC-specific β-arrestin suppression by RNAi abolishes the longevity phenotype (B,C) and the stress resistance phenotype (D) in both sexes. Control 1 = w1118 x Dilp2-Gal4, Control 2 = w1118 x UAS-mth22, Control 3 = w1118 x UAS-β-arrestinRNAi, mth22;β-arrestinRNAi = Dilp-2-Gal4 x UAS-m22-β-arrestinRNAi. Overexpression of β-arrestin alone in the IPC’s (E) also extends life in both sexes (F,G). Relative abundance of all RT-PCR is shown have been normalized to control that is one. Control 1 = w1118 x Dilp2-Gal4, Control 2 = w1118 x UAS-β-arrestin, β-arrestin = Dilp2-Gal4 x UAS-β-arrestin.