Figure 7.

Test pollinations for evaluation of SLG and SRK transgenic plants by seed formation. Line S⁶⁰ was used as the recipient of the transgene and line S²⁸S⁶⁰ was used as the pollen tester. In this case, the S phenotype of all pollen was S²⁸ because S²⁸ is dominant over S⁶⁰. The phenotype of transformants carrying SLG²⁸ was coincident to that of the non-transformant recipient plant, that is S⁶⁰. In contrast, the seed formation of transformants having SRK²⁸ decreased compared to that of transformants having SLG²⁸ and recipient S⁶⁰ line. After genetic crossing of the two transformants, which carried SLG²⁸ and SRK²⁸ transgenes, the F₁ plant showed a rigid SI phenotype, like the S⁶⁰S²⁸ heterozygote. SI phenotype was assessed by the number of seeds formed per pod.