Table 1.
The functional changes in the electron transport chain in mitochondria under starvation of CL
| Strain | Temperature | Oxygen consumptiona | NADH oxidaseb | NADH-Q reductaseb | |||||
|---|---|---|---|---|---|---|---|---|---|
| −DBH | +DBH (50 µM) | ||||||||
| ℃ | nmol/min/107 cells | % | nmol/min/mg | % | nmol/min/mg | % | nmol/min/mg | % | |
| CHO-K1 | 33 | 8.0 ± 0.4 | 100 | 24.0 ± 3.2 | 100 | 6.7 ± 0.7 | 100 | 28.9 ± 6.2 | 100 |
| PGS-S | 33 | 10.1 ± 0.5 | 126 | 16.7 ± 1.2 | 70 | 6.1 ± 1.3 | 91 | 37.9 ± 1.6 | 131 |
| CHO-K1 | 40 | 6.5 ± 0.1 | 100 | 16.5 ± 2.4 | 100 | 6.2 ± 1.7 | 100 | 35.4 ± 2.8 | 100 |
| PGS-S | 40 | 4.1 ± 0.1 | 63 | 6.8 ± 1.8 | 41 | 1.3 ± 1.1 | 21 | 32.5 ± 4.2 | 92 |
| R6 | 40 | 5.5 ± 0.2 | 85 | 14.6 ± 0.2 | 88 | 4.5 ± 0.2 | 73 | NDc | |
a) Oxygen consumptions are measured by the intact cells. b) NADH oxidase and NADH-ubiquinone reductase activities are assayed by using the cell extracts of CHO-K1, PGS-S and R6 cells. c) Not determined.