Table 2.
qRTPCR Assay Conditions
A. Multiplexed Assay
| |
---|---|
Component | Concentration |
Beta forward primer | 150 nM |
Beta reverse primer | 200 nM |
Beta probe | 200 nM |
Alpha forward primer | 100 nM |
Alpha reverse primer | 150 nM |
Alpha probe | 200 nM |
MgCl2 | 4.5 mM |
B. GRcommon Assay
| |
---|---|
Component | Concentration |
Common forward primer | 100 nM |
Common reverse primer | 200 nM |
Common probe | 300 nM |
MgCl2 | 4.5 mM |