Table 3.

Rat GR PCR Primers and Expected PCR Products

A: PCR PRIMERS
Primer Designation	Sequence	Position	Location
F1	TACCACAGCTCACCCCTACC	RefSeq 1638–1657	Exon 4
F2	ATAAAAGCCTGAGGGGAGGA	RefSeq 101–124	Exon 1
F3	GATGACCAAATGACCCTGCT	RefSeq 1832–1851	Exon 5
F4	ACGGGACCACCTCCCAAGCTC	RefSeq 1307–1327	Exon 2
F5	GTCAGAACTGGCAACGGTTT	RefSeq 2190–2209	Exon 7
F6	CCACTGCAGGAGTCTCACAA	RefSeq 1575–1594	Exon 4
F7	AGTGCCACATGCCTTTAACC	Intron7 57–76	Intron 7
ALPHA FOR	GAGTCTCACAAGACACTTC	RefSeq 1584–1602	Exon 4
R1	CCCATGTTTCTGCCTCTTTC	Intron 7 192–211	Intron 7
R2	GGTTAAAGGCATGTGGCACT	Intron 7 57–76	Intron 7
R4	ATCCAGCATTTGGGAAACTG	Intron 7 523–542	Intron 7
R5	GCACTGCCCACAACCGAGTTTT	Intron 7 40–61	Intron 7
R6	TCATGCTTTATTGGCCTTCA	Intron 7 682–701	Intron 7
R7	CTCAGGAATCCCACAAAAACA	Intron 7 755–775	Intron 7
R8	ATTATGGCATCCCCTCCTTC	Intron 7 958–977	Intron 7
R9	AGCAAAGCAGAGCAGGTTTC	RefSeq 1917–1936	Exon 5
ALPHA REV	CAGCTAACATCTCTGGGAATTC	RefSeq 2306–2327	Exon 8

B: Expected Products
Primer pair	Location	Predicted size	Sequenced
F1 R1	Exon 4 to Intron 7 (211)	819	yes
F1 R2	Exon 4 to Intron 7 (76)	684	yes
F2 R2	Exon 1 to Intron 7 (76)	2217
F3 R4	Exon 5 to Intron 7 (542)	942	yes
F4 R5	Exon 2 to Intron 7 (61)	1000	yes
F5 R6	Exon 7 to Intron 7 (701)	743
F7 R7	Intron 7	705
F7 R8	Intron 7	907
F6 R9	Exon 4 to Exon 5	362
AlphaF AlphaR	Exon 4 to Exon 8	744	yes