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. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: Neurogastroenterol Motil. 2012 Dec 18;25(2):e89–e100. doi: 10.1111/nmo.12057

Figure 2.

Figure 2

Transduction of lumbosacral and thoracolumbar DRG by rAAV8-GFP after intrathecal vector delivery between the L5/L6 vertebrae. A, Gradient of transduction in lumbosacral (L5 and L6) and thoracolumbar (T13 and L1) DRG (n = 6; shown are mean +/− SE). B, Attenuation of caudal-to-rostral diffusion of cerbrospinal fluid increased transduction in L6 DRG (t-test, P < 0.05). The mice in group “VERTICAL” (n = 3) were injected intrathecally under anesthesia and positioned approximately vertically (head upward) for the duration of the anesthesia (approximately 1 h). The mice in group “CONTROL” (n = 5) were awake during the injection and allowed to move freely after it. C, Neuronal GFP-ir in the L6 DRG showing highest level of transduction in B. Scale bar: 50 μm.