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. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: Neurogastroenterol Motil. 2012 Dec 18;25(2):e89–e100. doi: 10.1111/nmo.12057

Figure 3.

Figure 3

GFP-ir varicose networks within the myenteric plexus. All images show GFP in green, CGRP in red, and SP in blue. A–D, Triple labeling for GFP, CGRP, and SP in a section through a folded region of the colon sample. Images (projections of 5 slices 1 μm apart) show a nearly horizontal view of the myenteric plexus overlaying the longitudinal muscle in the center and bordering the circular muscle (CM) at each side. E–H, Triple labeling for GFP, CGRP, and SP in a myenteric ganglion within a transverse colon section in which the CM is located above the ganglion. GFP-ir nerve fibers have a branching varicose appearance and form a diffuse network within the myenteric ganglia (A, E). GFP-ir overlaps substantially with CGRP-ir as indicated in B and F by the merging of green and red into orange and yellow. Colocalization of GFP- and SP-ir nerve fibers is limited (C, G). Scale bars: A–D, 20 μm; E–H, 10 μm.