Figure 5. Integrating multiple optical tools for complex control.
In this hypothetical scheme, the phyB/PIF optical dimerizer system is combined with a CRY1/phyB system that is light-dissociated. Thus, in blue light, none of the proteins interact. In dark or far-red light, phyB interacts with CRY1, while in red light phyB interacts with PIF. Thus, a protein of interest tethered to phyB can be shuttled from one location to the other (for example, plasma membrane vs. nuclear membrane) via changes in light wavelength.