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. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: J Immunol. 2013 Jan 2;190(3):977–986. doi: 10.4049/jimmunol.1201331

Figure 1. CD4+ T cells and CD4+Foxp3+ T cells accumulate in parallel after VZV challenge in the skin.

Figure 1

Healthy young volunteers were injected with 0.02ml VZV skin antigen test and 5mm punch biopsies were performed on days 0, 1, 3 or 7 post injection (with 4–7 volunteers per time point). (A) 6µm frozen skin sections were stained for CD4 (green) and Foxp3 (red) using an indirect immunofluorescence method (original magnification: x400). Cell numbers were expressed as the mean absolute number of cells counted within the frame. Graph shows numbers of total CD4+ cells (white squares) and numbers of CD4+Foxp3+ cells (black squares) in perivascular infiltrates (PV) following VZV skin antigen injection. (B) Graph shows the proportion of total CD4+ cells expressing Foxp3 in perivascular infiltrates before and after VZV challenge. Each symbol represents a mean of n=4–7 individuals per time point; average of 5 perivascular infiltrates counted for each individual. (C) The number of infiltrating CD4+ T cells in the skin correlates with clinical response (linear regression analysis, p<0.0001).