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. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: J Immunol. 2013 Jan 2;190(3):977–986. doi: 10.4049/jimmunol.1201331

Figure 3. VZV-specific CD4+ T cells in the skin predominantly have a central memory phenotype.

Figure 3

Skin suction blisters were induced on day 7 following VZV injection. PBMCs and blister cells were stimulated with VZV lysate for 15hrs in the presence of Brefeldin A and stained for intracellular expression of IFN-γ, IL-2, CD27 and CD45RA. (A) Representative FACS plots show expression of CD27 and CD45RA on total CD4 or IFN-γ secreting CD4+ T cells in the skin suction blisters and blood. Graph shows cumulative data (n=12) for total CD4 (black symbols) and IFN-γ+ CD4+ T cells (VZV- specific, white symbols) in the skin. (B) Representative FACS plots and cumulative data showing expression of CD27 and CD45RA on total and IL-2 secreting CD4 T cells (n=7).