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. Author manuscript; available in PMC: 2014 Mar 1.
Published in final edited form as: Synapse. 2012 Dec 8;67(3):145–159. doi: 10.1002/syn.21626

Figure 3.

Figure 3

Selective CB2 receptor antagonists prevent selective cannabinoid agonist-induced upregulation of 5-HT2A receptors in neuronal cells (A) Effect of antagonists of CB1 and CB2 receptors on the CP 55,940-induced upregulation of 5-HT2A receptor mRNA. JTE 907 pretreatment, a CB2 receptor antagonist, prevented the 5-HT2A receptor upregulation in CP 55,940-treated cells. PF514273 pretreatment, a CB1 receptor antagonist, did not modify the effect of CP 55,940-treatment on 5-HT2A mRNA levels. **p<0.01, significant effect of CP 55,940 on 5-HT2A receptor mRNA levels compared with vehicle-treated cells. ##p<0.01, significant effect of JTE 907 pretreatment on the CP 55,940-induced upregulation of 5-HT2A mRNA. (B) Effect of CP 55,940 (nonselective cannabinoid agonist), ACEA and GP 1a, selective CB1 and CB2 receptor agonists, respectively, on 5-HT2A receptor mRNA levels in CLU213 and A1A1v cells. **p<0.01, significant effect of GP 1a or CP 55,940 treatment on 5-HT2A receptor mRNA levels compared with vehicle-treated CLU213 or A1A1v cells. #p<0.05, significant effect of GP 1a or CP 55,940 treatment compared to ACEA treatment on 5-HT2A receptor mRNA levels in CLU213 or A1A1v cells. (C) JTE 907 and AM 630, selective CB2 receptor antagonists, prevented the GP 1a-induced upregulation of 5-HT2A receptor mRNA. **p<0.01, significant effect of GP 1a treatment on 5-HT2A receptor mRNA levels compared to vehicle treated cells. ##p<0.01, significant effect of JTE 907 or AM 630 pretreatment on the GP 1a-induced upregulation of 5-HT2A receptor mRNA in CLU213 cells. The data represent mean ± SEM (n=3).