Figure 4.
Effect of delaying initial access to ethanol self-infusion during withdrawal (Experiment 2). (A) Ethanol-dependent mice received daily passive infusions of ethanol for 5 days (P1-P5) as shown in Figure 1; non-dependent water control mice received matched passive infusions of water (not shown in design). Delays of 0, 1, 3 or 5 days separated the last passive infusion session from the first no-choice self-infusion session for Groups 0, 1, 3 and 5, respectively. (B) Mean ethanol intakes (g/kg/d) during the no-choice self-infusion phase. Data were averaged over 2 no-choice sessions (n = 7-13/group). *Significantly different from all other groups within the same strain, Bonferroni-corrected p's < .005; ∧ significantly different from Groups 3 and 5 within the same strain, Bonferroni-corrected p's < .001; ∨ significantly different from Water group, Bonferroni-corrected p < .05 (C) Mean ethanol intakes (g/kg/d) during the choice self-infusion phase were averaged over 5 sessions (n = 7-12/group). *Significantly different from all other groups within the same strain, Bonferroni-corrected p's < .005. (D) Mean S+ preference ratios (S+ licks divided by total of S+ and S- licks) during the choice self-infusion phase. *Significantly different from all other groups within the same strain, Bonferroni-corrected p's < .005; #significantly different from S+ preference ratio = 0.50 (dashed line, no preference), Bonferroni-corrected p's < .05. Error bars depict SEM.