Skip to main content
. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: Steroids. 2012 Nov 23;78(2):137–146. doi: 10.1016/j.steroids.2012.10.018

Figure 2.

Figure 2

Effect of CRAC treatment on aortic atheroma formation and associated oxidative stress in guinea pigs fed with high cholesterol diet. Hartley male guinea pigs were fed with standard or 2% cholesterol enriched diet for 14 weeks. CRAC treatment started at the end of week 8 until the end of the experiment. Guinea pig received one i.p. injection of CRAC solution at 3 and 30 mg/kg (2.5 ml/kg) or vehicle every other day. At the end of week 14, aortas were collected for histology, lipid histochemistry and for nitrosylated proteins immunofluorescence. This subset of experiments has been conducted on transverse section of aorta, 3 animals per group, 5 sections were analyzed per animal, and the most representative was used for illustration purpose. A–C. Standard diet. D–F. 2% cholesterol diet. G–I. 2% cholesterol diet treated with CRAC 3 mg/kg. J–L. 2% cholesterol diet treated with CRAC 30 mg/kg. A–B, D–E, G–H, J–K. Oil Red O staining and Mayer’s hematoxylin counterstaining. C, F, I, L. Primary antibody: anti-nitrotyrosine; secondary antibody: Alexa Fluor 488 conjugated anti-mouse.