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. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: J Immunol. 2012 Dec 28;190(3):1264–1275. doi: 10.4049/jimmunol.1202012

Fig. 6. ROS production was lower in MKK3 −/− mice and endothelial cells after LPS.

Fig. 6

a) WT and MKK3 −/− mice were given IP LPS (40mg/ml) and malondialdehyde (MDA) levels were measured in serum (n=5–8, Mean +/− SEM are shown, *p<0,05)

b) WT and MKK3 −/− lung endothelial cells were exposed to LPS (1μg/ml). Cells were stained with CM-H2DCFDA, which detects H2O2, levels were measured by flow cytometry. The values are expressed as mean fluorescent intensity +/− SEM (p<0.05). The results are representative of at least 3 independent experiments. Representative fluorescence histograms of LPS exposed cells are shown.

c) WT and MKK3 −/− lung endothelial cells were exposed to LPS (1μg/ml). Cells were stained with Mitosox red, which detects mitochondrial ROS, levels were measured by flow cytometry. The values are expressed as mean fluorescent intensity +/− SEM (p<0.05). The results are representative of at least 3 independent experiments. Representative fluorescence histograms of LPS exposed cells are shown.