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Fluorescence characterization of PANP. Before irradiation, PANP only showed detectable fluorescence in the VT680 channel (inset, excitation wavelength = 670 nm). Irradiation with long wavelength ultraviolet light lead to an increase in fluorescein fluorescence. A time course of the photo-uncaging process was monitored by fluorescence spectroscopy. As the photochemical reaction continued, an increase in fluorescence (excitation wavelength = 470 nm) was observed over time.
