FIG. 3.

Transcriptional activity of patient LTRs under basal and Tat-activated conditions in astrocytes and T cells. Matched CNS- and non-CNS-derived LTRs were analyzed for their transcriptional activity under basal (A, B) and Tat-activated conditions (C, D). Basal conditions were assessed in both a human fetal astrocyte cell line (SVG, A) and a T cell line (Jurkat, B), and results are shown relative to the wild-type NL4-3 LTR. Similarly, Tat-activated conditions were also assessed in SVG (C) and Jurkat (D) cell lines using saturating amounts of HXB2 Tat, and results are shown as fold activation over basal. CNS-derived LTRs are shown in black and non-CNS LTRs are shown in gray. Error bars represent mean±standard error (SE). The data shown are a compilation of the means of four independent experiments performed in triplicate. Statistical analyses were performed using the Mann–Whitney U-test with p values<0.05 considered statistically significant.