Figure 4. Fluorescence immunocytochemistry for CD3, CD22 and CD14 with slice labeled by FR.

Red fluorescence (A1,B1,C1) is labeled nerve fibers, and green fluorescence (A2,B2,C2) is CD3⁺,CD22⁺and CD14⁺cells. A3, B3 and C3 are respectively merged images from CD3⁺ ,CD22⁺ and CD14⁺ cells and FR-labeled nerve fibers. The merged images showed that labeled nerve fibers were not around CD3⁺, CD22⁺ and CD14⁺ cells (A3, B3, C3 and A4, B4, C4). This immunofluorescence results demonstrated that the nerve fibers from superior cervical ganglion do not target T cells, B cells and macrophages.