Table 1. Strains and plasmids used in this study.

Strain or plasmid	Genotype/comments	Source or reference
Strains
S. coelicolor A3(2)
M145	Prototrophic, SCP1− SCP2−	Kieser et al., 2000
SLC201	M145 derivative with a deletion of the mce operon (see Fig. 1.)	This work
ΔmtrA	M145 ΔmtrA::apr	This work
Plasmids
pIJ10702	Cosmid backbone replacement integrating at phage φC31 attB site.	Foulston and Bibb, 2010
pNRT4	Kanamycin (aphII) containing E coli shuttle vector	Herron, University of Strathclyde, Unpublished
pLC006	Cosmid 8A2 digested to 22 kbp, with the Supercos1 backbone swopped with pIJ10702 and pNRT4 ligated to confer kanamycin resistance for counter selection.	This work