Table 1.
T-DNA vector | transformantsa (per 5×107 yeast) |
vector backbone carryoverb |
---|---|---|
pCM41 | 14.0 ± 6.8 (n=5) | 12.5% (n=32) |
pBS03 | 87.2 ± 24.5 (n=5)* | 75.0% (n=40) |
pBHt2 | 79.5 ± 20.2 (n=5)* | 8.3% (n=60) |
average ± SD (n=number of experiments), significant difference from pCM41 (P < 0.01) by one-tailed t-test indicated by asterisks
carryover frequency determined by positive aph PCR (n=number of transformants analyzed)