Table 2.

Characteristics of T-DNA integration in Histoplasma

			numbera
Left Border (LB)	TAIL-PCRb	59	(87%)
	backbone carryoverc	2	(3%)
	truncation (base pairs)d	0 – 45	(9.0 ± 9.1)
Right Border (RB)	TAIL-PCRb	64	(94%)
	backbone carryoverc	6	(9%)
	truncation (base pairs)d	0 – 8	(0.4 ± 1.2)
integration contexte	single copy DNA	101	(86%)
	repetitive DNA	16	(14%)
	hotspotsf	0	(0%)
integration characteristicsg	simple integrationsh	33	(67%)
	large deletionsi	1	(2%)
	genome rearrangementsj	15	(31%)

^anumbers represent the number of mutants and % of total characterized except for truncation numbers which represent the range and mean number of base pairs deleted

^bsuccessful generation of TAIL-PCR products with sequencing anchored within the T-DNA element

^cT-DNA insertion includes vector sequence beyond the border end

^dnumber of nucleotides deleted from the T-DNA left or right border: range (mean ± SD)

^eLB and RB flanking sequences considered individually (n=117)

^fintegration hotspots defined as insertions within 5,000 base pairs of another insertion

^gintegration characteristics determined for 49 mutants for which both LB and RB-flanking genome sequence was obtained

^hintegrations in which genome sequence flaking the LB and RB match the same contig and are within 1000 base pairs of each other

ⁱintegrations in which genome sequence flaking the LB and RB match the same contig but are separated by more than 1000 base pairs

^jintegrations in which genome sequence flaking the LB and RB do not match the same contig or are separated by at least 1,000 base pairs on the same contig and PCR tests confirm no loss of intervening genome sequence