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. Author manuscript; available in PMC: 2014 Jan 16.
Published in final edited form as: Cell Host Microbe. 2013 Jan 16;13(1):54–66. doi: 10.1016/j.chom.2012.10.021

Figure 7. The effect of axonal gene knockdown on retrograde PRV infection.

Figure 7

(A) Images show representative areas in the S-compartments. N-chambers were transfected with either siNT or gene-specific siRNAs before PRV 233 infection. DiI stained cell bodies (a–d), GFP positive cell bodies (e–h), merged images (i–l), and phase contrast images (m–o) are shown. (B) Graph depicts the percentage of GFP positive to DiI positive cell bodies of either siNT or gene-specific siRNA transfected axons. Data shown are the mean of 3 independent experiments consisting of duplicates. Data are the mean ± SEM with *P<0.05,**P<0.01 and ***P<0.001 using one sample t-test. (C) Hypothesized cascade of events that take place in retrograde PRV infection of healthy and injured axons. 1. Virion attachment and entry, increase in cytosolic calcium and phosphorylation events 2. Translation of axonal mRNAs 3. Formation of dynein coupled transport complexes. 4. Beta-importin dependent nuclear localization and subsequent transcription and replication of viral genomes.