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A) Time-dependent intensity ratios (green/red emission) of HA-FRET probe in the absence and presence of HA-ase. The concentration of HA-FRET sample (0.25 ml) was 1 μM, the excitation was 470 nm, and the experiment was done at room temperature. B) The dependence of HA-FRET probe's green/red intensities on the HA-ase concentration. The concentration of HA-FRET probe (0.25 ml) was 1 μM, and the incubation time at room temperature was 1 hour.
