Table 1.
Assay of GFP fluorescence as an indicator of integrase mediated gene insertion. The table indicates starting numbers of one cell embryos, the number of trials, survivorship to stage 46 and the number of embryos with GFP fluorescence at stage 46.
| Sample | # Embryos Injected (#trials/mothers) | Stage 46 Survival | Stage 46 Fluoresence | Avg % of embryos glowing (Range) |
|---|---|---|---|---|
| Non-Injected | 1194(12/7) | 779 | 0.00E+00 | 0.00E+00 |
| DEPC H2O | 916 (7/5) | 398 | 0.00E+00 | 0.00E+00 |
| Integrase mRNA 1 ng | 125 (1/1) | 44 | 0.00E+00 | 0.00E+00 |
| 5 pg pEGFPB2 | 337 (3/3) | 165 | 0.00E+00 | 0.00E+00 |
| 5 pg insulated pEGFPB2 plasmid | 300 (4/3) | 118 | 0.00E+00 | 0.00E+00 |
| 5 pg pEGFPB2 plasmid + 1 ng Integrase | 452 (3/3) | 258 | 95 | 36.82 (12-49) |
| 5 pg insulated pEGFPB2 plasmid + 1 ng integrase | 300 (4/3) | 148 | 47 | 31.76 (29-44) |
| 5 pg insulated pEGFPB2 plasmid + 500 pg integrase | 100(1/1) | 34 | 4 | 11.76 |
| 5 pg insulated pEGFPB2 plasmid + 50 pg integrase | 100(1/1) | 52 | 2 | 3.85 |
| 5 pg insulated crystallin lens plasmid | 200 (4/2) | 69 | 0.00E+00 | 0.00E+00 |
| 5 pg insulated crystallin lens plasmid + 1 ng Integrase | 200 (4/2) | 73 | 29 | 39.73 (37-44) |