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. Author manuscript; available in PMC: 2013 Jan 23.
Published in final edited form as: Methods Mol Biol. 2013;965:185–196. doi: 10.1007/978-1-62703-239-1_12

Fig. 1.

Fig. 1

IMR90 cells were infected with a puromycin-resistant retrovirus encoding oncogenic H-RASG12V or vector control. Drug-selected cells were stained with antibodies to H3K9Me2 and histone H2A variant macroH2A. DAPI counterstaining was used to visualize SAHF. Note the robust punctate DAPI foci in the H-RASG12V infected cells, which co-localize with both macroH2A and H3K9Me2. Arrows point to inactivated X chromosome in control IMR90 female fibroblast cells. Chicken anti-macroH2A1.2 primary antibody was obtained from Dr. John R. Pehrson (University of Pennsylvania) (28). Rabbit anti-H3K9Me3 is from AbCam (ab8898) and was used at 1:500. Secondary antibodies were FITC-labeled goat anti-chicken (1:2500) and Cy3-labeled goat anti-rabbit (1:5000), which were both obtained from Jackson Immunolabs.