Figure 3. Binding properties of monomeric and dimeric forms of anti-hFcRn Nb218-H4.

Serial dilutions of Nb218-H4 injected over immobilized hFcRn at either pH 7.4 or pH 6.0. The equilibrium binding responses obtained at (A) pH 7.4 and (B) pH 6.0 were fitted to a steady state affinity binding model supplied with the BIAevaluation software. (C) A representative sensorgram showing the overlays of serial dilutions of Nb218-H4 injected over immobilized hFcRn at pH 7.4. The derived binding kinetic values are presented in Table 1. (D) A representative sensorgram showing binding of equal amounts of monomeric Nb218-H4 and bivalent (Nb218-H4)₂ to immobilized hFcRn at pH 6.0. Injections were performed at 25°C with a flow rate of 50 μl/min. n = 3–4. All data are presented as mean±s.d.