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. Author manuscript; available in PMC: 2013 May 16.
Published in final edited form as: Science. 2012 Nov 16;338(6109):949–953. doi: 10.1126/science.1227157

Fig. 2. Seeded α-Syn pathology leads to progressive DA system degeneration.

Fig. 2

(A–D) pSyn immunostaining in SNpc of mice sacrificed at 30d, 90d, or 180d following striatal PFF injection. (A) Diffuse perinuclear pSyn inclusions (black arrowheads) at 30d post-injection. (B,C) Dense LB-like inclusions (black arrows) at 90d and 180d post-injection. Absence of pSyn pathology in SNpc contralateral to the PFF injection site (D) and in ipsilateral SNpc of PBS-injected mouse (E) at 180d. (F–I) TH-immunostaining of SNpc at 30d, 90d and 180d following inoculation with Syn PFFs. Arrowheads in (G) indicate neurons with reduced TH staining in the ipsilateral SNpc at 90d post-injection. (H,I) Ventral SNpc, ipsilateral and contralateral to the site of PFF injection at 180d. Arrows point to DA neuron loss. (J) PBS-injected control at 180d post-injection. (K) Percentage of SNpc TH-neurons containing pSyn-immunoreactive inclusions for each treatment group. Data for treated ipsilateral (black) and contralateral (grey) hemispheres are shown. #p<0.001 paired t-test (N = 3–5 animals per group). (L,M) Quantification of TH-immunoreactive neurons in the SNpc and VTA of mice after intrastriatal PFF-, monomer- or PBS-injection. Data represent mean number of cells per region +/− SEM (N = 3–4 animals per group). *P<0.05 one-way ANOVA. Scale bars: 25 μm (A–D);50 μm (E–J).