Fig 4.
Mechanism of inhibition by α-syn. A, GCase (10 nM) reaction velocity as a function of added 4MU-β-glu in the presence of 0 μM (●), 0.15 μM (■), 0.3 μM (▲) and 1.25 μM (▼) α-syn. Fits to Michaelis-Menten equation for mixed inhibition are shown as lines. B, Plot of apparent Vm(app) vs. Km(app) extracted from fits.