FIG. 3. Anisomycin, SG and ricin differentially activate p38, JNK and ERK.
Cells were treated with anisomycin (25 ng/ml), SG (10 ng/ml) and ricin (300 ng/ml) for 0.5, 1, 2, 3, 4, 5 and 6 h, and DON (1000 ng/ml) for 0.5 h, respectively. Cells were then lysed and subjected to Western blotting analysis with total and phosphorylated (A) p38, (B) JNK and (C) ERK antibodies. β-actin was immunochemically stained as loading control (A).