Table 1.
Theoretical m/z values of human IgG Fc glycopeptides detected by nano-LC-ESI-MS
| Glycan species | IgG1 P01857b | IgG2/3 P01859b/P01860, VAR_003892b | IgG4 P01861b | |||
|---|---|---|---|---|---|---|
| [M + 2H]2+ | [M + 3H]3+ | [M + 2H]2+ | [M + 3H]3+ | [M + 2H]2+ | [M + 3H]3+ | |
| G0Fc | 1317.527 | 878.687 | 1301.532 | 868.024 | 1309.529 | 873.356a1 |
| G1F | 1398.553 | 932.705 | 1382.558 | 922.042 | 1390.556 | 927.373a2 |
| G2F | 1479.580 | 986.722 | 1463.585 | 976.059 | 1471.582 | 981.391 |
| G0FN | 1419.067 | 946.380 | 1403.072 | 935.717 | 1411.069 | 941.049a3 |
| G1FN | 1500.093 | 1000.398 | 1484.098 | 989.735 | 1492.096 | 995.066a4 |
| G2FN | 1581.119 | 1054.416 | 1565.125 | 1043.752 | 1573.122 | 1049.084 |
| G1FS | 1544.101 | 1029.737 | 1528.106 | 1019.073 | 1536.104 | 1024.405a5 |
| G2FS | 1625.127 | 1083.754 | 1609.133 | 1073.091 | 1617.130 | 1078.423 |
| G1FNS | 1645.641 | 1097.430 | 1629.646 | 1086.767 | 1637.643 | 1092.098 |
| G2FNS | 1726.667 | 1151.447 | 1710.672 | 1140.784 | 1718.670 | 1146.116 |
| G0 | 1244.498 | 830.001 | 1228.503 | 819.338 | n.d. | n.d. |
| G1 | 1325.524 | 884.019 | 1309.529 | 873.356a1 | n.d. | n.d. |
| G2 | 1406.551 | 938.036 | 1390.556 | 927.373a2 | n.d. | n.d. |
| G0N | 1346.038 | 897.694 | 1330.043 | 887.031 | n.d. | n.d. |
| G1N | 1427.064 | 951.712 | 1411.069 | 941.049a3 | n.d. | n.d. |
| G2N | 1508.090 | 1005.730 | 1492.096 | 995.066a4 | n.d. | n.d. |
| G1S | 1471.072 | 981.051 | 1455.077 | 970.387 | n.d. | n.d. |
| G2S | 1552.098 | 1035.068 | 1536.104 | 1024.405a5 | n.d. | n.d. |
| G1NS | 1572.612 | 1048.744 | 1556.617 | 1038.081 | n.d. | n.d. |
| G2NS | 1653.638 | 1102.761 | 1637.643 | 1092.098 | n.d. | n.d. |
a1–a5 isomeric glycopeptide species of IgG4 and IgG2
b SwissProt entry number
c glycan structural features are given in terms of number of galactoses (G0, G1, G2), fucose (F), bisecting N-acetylglucosamine (N), and N-acetylneuraminic acid, sialic acid (S)
n.d. not detected