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A. X-ray scattering of a 28.2 kDa RNA at 1 mg/ml concentration from either a synchrotron source (Advanced Photon Source or APS) or bench top source (Bruker Nanostar) for 4 hours (see Basic Protocol 2). Synchrotron data were averaged over a total of 3 min. from 4 individual 45 sec. exposures. The bench top data were collected from a single 4 hour exposure. B. SAXS and WAXS data from the same RNA demonstrating accurate and inaccurate buffer subtraction (see Basic Protocol 3).
