Table 4.
GO analysis
| Term | Count | % | p-Value | Fold | Benjamini |
|---|---|---|---|---|---|
| Biological process | |||||
| Transport | 57 | 27.67% | 1.40 × 10−08 | 2.10779601 | 7.27 × 10−05 |
| Establishment of localization | 57 | 27.67% | 3.74 × 10−08 | 2.0512741 | 9.71 × 10−05 |
| Localization | 60 | 29.13% | 1.46 × 10−07 | 1.91908255 | 2.53 × 10−04 |
| Ion transport | 25 | 12.14% | 1.50 × 10−06 | 3.08118005 | 0.001943 |
| Synaptic transmission | 12 | 5.83% | 1.16 × 10−05 | 5.49530653 | 0.011935 |
| Chloride transport | 7 | 3.40% | 4.92 × 10−05 | 10.5684476 | 0.041641 |
| Transmission of nerve impulse | 12 | 5.83% | 6.38 × 10−05 | 4.56499873 | 0.04624 |
| Exocytosis | 8 | 3.88% | 6.71 × 10−05 | 7.88782093 | 0.042589 |
| Cation transport | 17 | 8.25% | 1.41 × 10−04 | 3.04192354 | 0.07808 |
| Metal ion transport | 15 | 7.28% | 1.72 × 10−04 | 3.30910296 | 0.085535 |
| Cell–cell signaling | 13 | 6.31% | 4.67 × 10−04 | 3.37670829 | 0.197738 |
| Cellular component | |||||
| Synapse | 19 | 9.22% | 1.69 × 10−10 | 7.12724905 | 1.33 × 10−07 |
| Postsynaptic membrane | 12 | 5.83% | 3.96 × 10−08 | 9.73280098 | 1.55 × 10−05 |
| Synapse part | 12 | 5.83% | 1.25 × 10−07 | 8.71242669 | 3.27 × 10−05 |
| Cytoplasmic part | 66 | 32.04% | 1.11 × 10−06 | 1.75847144 | 2.17 × 10−04 |
| Cell junction | 18 | 8.74% | 2.18 × 10−06 | 4.05127841 | 3.42 × 10−04 |
| Cytoplasm | 91 | 44.17% | 1.52 × 10−05 | 1.44439091 | 0.00198 |
| Plasma membrane | 44 | 21.36% | 5.11 × 10−05 | 1.85625586 | 0.005706 |
| Molecular function | |||||
| Ion transmembrane transporter activity | 30 | 14.56% | 1.74 × 10−10 | 4.09234024 | 4.70 × 10−07 |
| Transporter activity | 42 | 20.39% | 4.86 × 10−10 | 2.91474846 | 6.58 × 10−07 |
| Substrate-specific transmembrane transporter | 31 | 15.05% | 1.15 × 10−09 | 3.65758191 | 1.04 × 10−06 |
| Transmembrane transporter activity | 33 | 16.02% | 2.22 × 10−09 | 3.36413152 | 1.50 × 10−06 |
| Substrate-specific transporter activity | 33 | 16.02% | 1.85 × 10−08 | 3.08048906 | 9.99 × 10−06 |
| Gated channel activity | 17 | 8.25% | 3.63 × 10−08 | 5.82250711 | 1.64 × 10−05 |
| Ligand-gated channel activity | 11 | 5.34% | 5.03 × 10−08 | 11.1421945 | 1.70 × 10−05 |
| Ligand-gated ion channel activity | 11 | 5.34% | 5.03 × 10−08 | 11.1421945 | 1.70 × 10−05 |
| Anion transmembrane transporter activity | 12 | 5.83% | 6.01 × 10−08 | 9.36542127 | 1.81 × 10−05 |
| Ion channel activity | 18 | 8.74% | 2.53 × 10−07 | 4.7344533 | 6.84 × 10−05 |
| Substrate specific channel activity | 18 | 8.74% | 3.85 × 10−07 | 4.59483135 | 9.47 × 10−05 |
| GO term analysis (top increase in DKO 14 genes) | |||||
| Biological process | |||||
| Vesicle-mediated transport | 3 | 21.43% | 0.02265 | 11.0206034 | 35.4494 |
| Molecular function | |||||
| Binding | 11 | 78.57% | 0.031126 | 1.41461519 | 1 |
| Transporter activity | 4 | 28.57% | 0.045036 | 4.34057779 | 1 |
| Protein binding | 9 | 64.29% | 0.005192 | 2.31262748 | 0.999999 |
GO (gene ontology) term analysis of transcripts up/down-regulated in WT vs DKO (see Figure 1A). Data of upper table are derived from top 215 down-regulated transcripts in DKO; (positive value in Figure 1A). Data of lower table are from top 14 up-regulated transcripts in DKO; (negative value in Figure 1A). Taken together, two tables suggested that NOVA regulates the levels of many transcripts relating to synaptic transmission (Dennis et al., 2003; Huang et al., 2009).