Figure 1. Characterization of purified GPIb-IX complex in Triton X-100.

(A) GPIb-IX complex was purified from human platelets and solubilized in 0.1% Triton X-100-containing buffer. The complex was resolved in a 10% SDS gel under both non-reducing (N.R.) and reducing (R.) conditions and detected by Coomassie blue staining. (B) The purified GPIb-IX complex was applied to a gel filtration column Superose 6 equilibrated with 0.1% Triton X-100-containing buffer. Protein elution was monitored by absorbance at 280 nm (A₂₈₀). The calibrated Stokes diameter scale and void volume for this column are shown at the top.