Figure 3. Probing GPIb-IX complex reconstituted in Nanodiscs by conformation-sensitive monoclonal antibodies.

1 μg/ml of mouse IgG or antibodies against GPIbα (SZ2, AK2 and AN51) or against GPIX (FMC25) was incubated with immobilized GPIb-IX/ND (■) or empty Nanodiscs ( ) or BSA (□). The bound antibodies were detected by ELISA, using HRP-conjugated goat anti-mouse antibody. Absorbance at 450 nm was measured from 3 independent experiments and presented as the mean ± SD.