Figure 3.

Two example image sets are shown to demonstrate the dependence of the observed biological effect on tumor tissue and the attachment of targeted microbubbles onto endothelium cells. (a–e) An example of regions of reduced blood flow using 4 MPa destruction pulses with LXY-3-conjugated microbubbles in NDL tumors. Compared to the pre-treatment images (a), additional regions with low echo intensity were observed in the tumor (d) after the bound microbubbles were destroyed (b, c). The echo intensity of bound microbubbles (e) decreased in regions where echoes from flowing agents were reduced (d). White arrows indicate regions of reduced contrast image intensity. (f–i) With the destruction of control non-targeted flowing microbubbles (g, h), no additional region of low blood flow was observed on CPS images of flowing microbubbles acquired before and after flowing microbubble destruction (f, i). The scale bar represents 5 mm.