Figure 5.

Summary of the effect of multiple targeted microbubble injections (a–c, g), the time required for vascular recovery (d–f, g). Multiple injections of LXY-3-conjugated microbubbles, each followed by insonation, increased the area with reduced blood flow (a–c). Tumor (arrows) images were acquired before microbubble destruction (a), after the first microbubble destruction (b) and after the second microbubble destruction (c). The area with reduced echo intensity increases significantly after each application of destructive insonation (g). Evaluating vascular recovery after insonation demonstrated that regions with low contrast intensity following microbubble destruction recovered ^~40 minutes after the first microbubble injection (d–f). LXY-3-conjugated microbubbles were injected at time 0, 12 and 40 minutes. Destructive ultrasound pulses were applied once at 7.5 minutes after the first injection and CPS tumor images (arrows) were acquired at 0.5 (d), 12.5 (e) and 40.5 minutes after the first injection (f). The area with reduced echo intensity increased after microbubble destruction but returned to the initial level after ^~30 minutes (g). * indicates p<0.05. The scale bar represents 5 mm.