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. 2013 Jan 18;13:20. doi: 10.1186/1471-2334-13-20

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Copyright ©2013 Ocadiz-Delgado et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Neuraminidase A (H1N1) expression levels in clinical samples. (a) Duplex RT-PCR of A (H1N1) Neuraminidase (NA) and β2-microglobulin (β2-m) in the eigth A (H1N1)-positive samples (P01-P08). In all experiments, two negative Influenza-like illness patients [Neg ILI (−) 1, Neg ILI (−) 2] and positive [Pos ILI (+); H1N1 Puerto Rico/Puerto Rico/IvPR8/Puerto Rico; 98% homology] sample were included as controls. In addition, HeLa cervical cell line and a negative control with no sample (no cDNA) were included [HeLa and Neg (no cDNA), respectively]. RNA preparations, obtained from nasopharyngeal swabs of A (H1N1) positive cases were assayed using NA or β2-m specific primers (729 and 100 bp amplification product, respectively). MWM: Molecular weight marker. (b) Neuraminidase A (H1N1) expression levels determined in the same patient’s samples by quantitative RTqPCR. Viral load results are expressed as Relative Units.