Table 2.
List of primers used for the qPCR reactions
| Genes | Short name | Sequence 5' to 3' | Accession |
|---|---|---|---|
|
radical S-adenosyl methionine domain-containing protein 21 |
Rsad2 |
F-GTACCGCAGATGCACAACAC |
AF076620 |
| |
|
R-TTGACACTGCTTGGAGTTGC |
|
|
interferon inducible protein Gig11 |
Gig1 |
F-GGCAACCTGAATCCAGAAGA |
DW569595 |
| |
|
R-GTCTGGACGCAGACTGATGA |
|
|
VHSV-inducible protein1 |
Vhsv2 |
F-GGTGAAGACCTGGACCTGAA |
BT072288 |
| |
|
R-TGACCCCTGTTGACCTTCTC |
|
|
interferon-induced protein with tetratricopeptide repeats 51 |
Ifit5 |
F-CAGAGAGGTGCCAGGCTAAC |
BT046021 |
| |
|
R-TGCACATTGACTCTCCTTGG |
|
|
interferon induced with helicase C domain 11 |
Mda5 |
F-CAGAGGTGGGGTTCAATGAT |
NM001195179 |
| |
|
R-AGCTCGCTCCACTTGTTGAT |
|
|
DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide RIG-I1 |
Rig-I |
F-GACGGTCAGCAGGGTGTACT |
DY714827 |
| |
|
R-CCCGTGTCCTAACGAACAGT |
|
|
barrier-to-autointegration factor1 |
Baf |
F-ACAGACCCCTCATCATCCTG |
BT049316 |
| |
|
R-CGGTGCTTTTGAGAAGTGGT |
|
|
signal transducer and activator of transcription 1 isoform alpha1 |
Stat1a |
F-CGGTGGAGCCCTACACTAAG |
CB513054 |
| |
|
R-GGGATCCTGGGGTAGAGGTA |
|
|
interferon-inducible protein Gig2-like1 |
Gig2 |
F-GATGTTTCATGGCTGCTCAA |
BT044026 |
| |
|
R-CTTTTCGGATGTCCCGACTA |
|
|
B-type natriuretic peptide2 |
BNP |
F-TCGACAAATCCGCAATAAGA |
CK883650 |
| |
|
R-TTGAGCCAATTCGGTCTAGC |
|
|
putative collagen alpha 12 |
put_Coll-a1 |
F-AACCCTGAACCCCTCAGTCT |
CA038317 |
| |
|
R-TGGTCCTACCGTCTGGTTTC |
|
|
leukocyte elastase inhibitor2 |
LEI |
F-TCTCAGATGGCAAAGGCTCT |
BT045959 |
| |
|
R-GTTGGCCAGTTTCAGGATGT |
|
|
elongation factor 1a3 |
EF1α |
F-CACCACGGGCCATCTGATCTACAA |
BT072490 |
| |
|
R-TCAGCAGCCTCCTTCTCGAACTTC |
|
|
18S rRNA3 |
18S |
F-GCCCTATCAACTTTCGATGGTAC |
AJ427629 |
| |
|
R-TTTGGATGTGGTAGCCGTTTCTC |
|
|
infectious pancreatic necrosis virus_polyprotein4 |
IPNV |
F-CCGACCGAGAACAT |
AJ877117 |
| R-TGACAGCTTGACCCTGGTGAT |
1 Virus-responsive genes (Figure 4).
2 Genes used for microarray validation.
3 Reference genes used for normalization.
4 IPNV gene used for relative virus quantification (described in Results section).