Skip to main content
. 2013 Jan 23;8(1):e54708. doi: 10.1371/journal.pone.0054708

Figure 1. Schematic representation of chloroplast transformation vectors harboring tuberculosis candidate vaccine antigens.

Figure 1

Ls, Lactuca sativa; Nt, Nicotiana tabacum; pLD- vector components: the homologous Nicotiana tabacum chloroplast genome flanking sequence comprising of 16S 3′ end sequences and complete trnI, trnA genes; pLs- vector components: 16S trnI, the homologous Lactuca sativa chloroplast genome long flanking sequence containing 16S 3′end sequences and full length trnI gene; trnA 23S, trnA gene and 5′end of the 23S ribosomal RNA subunit; Prrn, ribosomal RNA operon promoter with GGAGG ribosome binding site; aadA, aminoglycoside 3′-adenylytransferase gene; TrbcL, 3′ untranslated region (Utr) of rbcL gene; PpsbA, promoter and 5′ Utr of psbA gene; TpsbA, 3′ Utr of psbA gene; CTB-ESAT6, coding sequence of cholera toxin B subunit fused to ESAT6 (6 kDa early secretory antigenic target); CTB-Mtb72F, coding sequence of cholera toxin B subunit fused to a recombinant fusion polyprotein from two TB antigens Mtb32 and Mtb39; LipY, coding sequence of a TB cell wall protein with lipase activity.